High quality
Diversified expression systems, scientific and reasonable screening system and optimization of expression protocols are performed to achieve highest yield expression and best sample quality.
Alfa Chemistry provides high quality membrane proteins services for global clients.
Membrane proteins play important functions, such as cellular communication and transferring materials in the cell. Moreover, many membrane proteins are involved in human diseases. To understand the function of these proteins, and even intending to alter them, requires a deep understanding of their complex structures. In this regard, a large amount of these proteins are required to be expressed for structural studies [1-2]. With our technology and expertise in membrane protein expressing, Alfa Chemistry has developed a variety of membrane protein expression systems to promote the study of its biological function and provide valuable information for drug discovery.
Alfa Chemistry has developed a variety of membrane proteins expression systems, including E. coli, Lactococcus lactis, yeasts, insect cells, mammalian cells and cell-free expression systems.
E. coli can be considered as the oldest and is still most popular host for the overproduction of membrane proteins. Its long tradition as a genetic model system resulting in a wealth of sophisticated and efficient genetic manipulation techniques and low cultivation cost.
Lactococcus lactis is an interesting alternative expression host. The expression system of membrane proteins based on Lactococcus lactis shows a variety of advantages, including easy and inexpensive cultivation, moderate proteolytic activity and efficient targeting of membrane proteins into the cytoplasmic membrane.
Single cells, fast growth rates, inexpensive media, high cell densities and the well-developed variety of genetic tools make yeasts to an attractive eukaryotic host. Yeasts have become the second most popular membrane proteins expression system after E. coli, with more cost effective than expression in mammalian or insect cell lines and often higher yields.
Cultivated insect cells in combination with vectors derived from the baculovirus family represent a major system for the expression of membrane proteins since 1980s.
Mammalian cells provide the most authentic cell environment for human membrane proteins, and they are able to perform specific post-translational modifications.
Cell-free expression offers promising new perspectives for the efficient production of membrane proteins for functional and structural applications, which is an emerging technique.
Six expression systems solve any problem you encounter with a specific membrane protein expression!
In addition to the complete expression systems, Alfa Chemistry optimizes the expression protocols for highest yield expression and best sample quality. Each little step, such as vector design and transfer, cultivation conditions, expression strategies, membrane proteins extraction, choice of detergents and buffer conditions are all within our optimization range.
With high-quality and reliable expression systems, our membrane protein expression service is an ideal choose for scientists or researchers to produce any specific membrane protein. Alfa Chemistry's membrane proteins expression service can produce various native and functional proteins, including but not limited to:
Diversified expression systems, scientific and reasonable screening system and optimization of expression protocols are performed to achieve highest yield expression and best sample quality.
Based on stable experimental system and rich experience, higher success rate of membrane proteins expression is achieved for many years to greatly save your time and cost.
According to requirements of customers and actual needs, the optimum combinational of expression experiment will be selected from different fusion tag proteins, expression vectors and expression hosts to form your customized solution.
20 L-1000 L membrane protein expression system can be performed that will provide you with milligram to gram protein.
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