A detailed understanding of the structure and biochemical and biophysical processes of membrane proteins is necessary for the advancement of life science and biomedical applications. Historically, the main barrier to membrane protein investigations has been the tendency of membrane proteins to aggregate (due to their hydrophobic nature) in aqueous solution as well as on surfaces. Nanodiscs provide a new and powerful tool for a broad spectrum of biochemical and biophysical studies of membrane proteins and are commonly acknowledged as an optimal membrane mimetic system for solubilizing and stabilization membrane proteins. Therefore, nanodiscs have been rapidly developed since their appearance and two major categories of membrane scaffold protein (MSP) nanodiscs and synthetic nanodiscs have been formed. Both MSP nanodiscs and synthetic nanodiscs are meant for the solubilization & stabilization of membrane proteins by mimicking a cell-membrane environment. So, the question is what type of nanodisc best suits your project? In general, each of the systems has its own unique characteristics, challenges and advantages. Alfa Chemistry compared the main differences between MSP nanodiscs and synthetic nanodiscs, and further summarized their advantages and disadvantages. This work may reduce your trial and error, save time for your research and help you choose the suitable nanodisc system.
Items | MSP Nanodiscs | Synthetic Nanodiscs |
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Stabilizing Belt | Membrane scaffold protein (such as MSP1D1, MSP1E3D1, MSP2N2, etc.) | Synthetic polymers (such as SMA, DIBMA, AASTY, AASTY, modified SMA, etc.) |
Lipid Composition | Artificial phospholipid (such as DMPC, DPPC, POPC.) | Native cell membrane phospholipids. |
Size | Depends on the used MSP protein size (Each MSP protein with uniform size (+/-1 nm)). Typically, 8-16 nm. | The size is variable depending on the length of the polymer chain. |
UV Absorption | Due to the presence of the MSP protein, the MSP nanodisc itself has ultraviolet absorption at a wavelength of 280 nm. | SMA based nanodiscs has ultraviolet absorption at a wavelength of 280 nm, but other synthetic nanodiscs such as DIBMA-based nanodiscs do not absorb at wavelengths of 280 nm. |
Nanodiscs Creation | There are three ways. | There's only one way. |
Involvement of Detergents | A detergent is needed to extract the membrane proteins from the cell membrane, firstly. | No detergent needed. |
Through the comparison of main differences between MSP nanodiscs and synthetic nanodiscs, we further make a brief summary of their advantages and disadvantages.
Items | MSP Nanodiscs | Synthetic Nanodiscs |
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Advantages | ||
Disadvantages |
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Alfa Chemistry is capable of producing a wide variety of MSP nanodiscs and synthetic nanodiscs related products, including MSP, SMA, DIBMA, AASTY, pre-assembled MSP nanodiscs and some kits. Meanwhile, we provide membrane proteins solubilization and stabilization service by using nanodiscs technology. These high-quality products and services will accelerate your membrane protein research, from membrane protein structure research, function research to application research. If you are interested in membrane proteins, please feel free to contact us for more details.
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