The MSP nanodisc is composed of α-helical-amphipathic proteins derived from apolipoprotein A-I, and phospholipid molecules, in which α-helical-amphipathic proteins wrap around the lipid bilayer as a homo dimer. These amphipathic proteins are commonly called membrane scaffold proteins (MSP), and they act as a belt around the lipid bilayer, holding them together and stabilizing the nanodisc. And their length determines the diameter of the nanodisc. As mentioned above, MSP is amphiphilic, and its structure contains a hydrophobic end towards the inner phospholipid layer and a hydrophilic end towards the outer layer. Such structure enables MSP nanodisc to have high solubility in aqueous solutions. Once the membrane protein is integrated into the MSP nanodisc, the membrane protein can maintain its monodisperse and activity in solution without detergent, which provides a strong technical support for membrane protein research. It can be seen that membrane scaffold protein (MSP) is the key component of nanodisc.
Fig. 1 Constructs of the first two MSPs used in MSP nanodisc (MSP1 and MSP2).
MSP1D1 is one of the most popular constructs, and is created by removing the first 11 N-terminal amino acids from MSP1 and replacing the 6×His tag and X linker with a 7×His tag and tobacco etch virus protease (TEV) linker to allow affinity tag removal. MSP1D1 can create assembled nanodiscs 9.5 nm in diameter. In addition, MSP1D1 can be modified further to create MSP1E1D1, MSP1E2D1, and MSP1E3D1 to enable nanodiscs with increased sizes (10.5 nm, 11.1 nm, and 12.1 nm in diameter, respectively). MSP2N2 and MSP2N3 represent other two popular constructs that can create assembled nanodiscs 16–17 nm in diameter. Smaller MSPs, MSP1D1ΔH4, MSP1D1ΔH5, MSP1D1ΔH4–5, and MSP1D1ΔH4–6, have also been designed to create nanodiscs with decreased sizes [1].
Fig. 2 Constructs of some typical MSPs.
Alfa Chemistry has developed a wide variety of MSPs with and without tags which can be assembled into tailor-made nanodisc of different sizes for the study of membrane proteins. Our MSP products can be divided into the following four types, each of which contains multiple products.
MSP1D1
MSP1D1 dH5 and MSP1D1-D73C
MSP1E3D1
MSP2N2
| Catalog | N-MNP-1 | N-MNP-2 | N-MNP-3 | N-MNP-4 | N-MNP-5 | N-MNP-6 |
|---|---|---|---|---|---|---|
| Product Name | Alpaca MSP1D1 with a his-tag | Mouse MSP1D1 with a his-tag | Human MSP1D1 with a his-tag | Rat MSP1D1 with a his-tag | Human MSP1D1, tag-free | Mouse MSP1D1, tag-free |
| Species | Alpaca | Mouse | Human | Rat | Human | Mouse |
| Tag | N-Terminal His-tag | N-Terminal His-tag | N-Terminal His-tag | N-Terminal His-tag | Tag-free | Tag-free |
| Scaffold Diameter | 9-10 nm | 9-10 nm | 9-10 nm | 9-10 nm | 9-10 nm | 9-10 nm |
| Purity | >90% | >90% | >90% | >90% | >90% | >90% |
| Form | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder |
| Available Size | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg |
| Shipping | Room Temperature | Room Temperature | Room Temperature | Room Temperature | Room Temperature | Room Temperature |
| Storage | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. |
| Catalog | N-MNP-7 | N-MNP-8 | N-MNP-9 |
|---|---|---|---|
| Product Name | Human MSP1D1 dH5 with a his-tag | Human MSP1D1 dH5, tag-free | Human MSP1D1-D73C with a his-tag |
| Species | Human | Human | Human |
| Tag | N-Terminal His-tag | Tag-free | N-Terminal His-tag |
| Scaffold Diameter | 7-8 nm | 7-8 nm | 9-10 nm |
| Purity | >90% | >90% | >90% |
| Form | Lyophilized powder | Lyophilized powder | Lyophilized powder |
| Available Size | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg |
| Shipping | Room Temperature | Room Temperature | Room Temperature |
| Storage | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. |
| Catalog | N-MNP-10 | N-MNP-11 | N-MNP-12 | N-MNP-13 | N-MNP-14 |
|---|---|---|---|---|---|
| Product Name | Mouse MSP1E3D1 with a his-tag | Human MSP1E3D1 with a his-tag | Rat MSP1E3D1 with a his-tag | Mouse MSP1E3D1, tag-free | Human MSP1E3D1, tag-free |
| Species | Mouse | Human | Rat | Mouse | Human |
| Tag | N-Terminal His-tag | N-Terminal His-tag | N-Terminal His-tag | Tag-free | Tag-free |
| Scaffold Diameter | 12-14 nm | 12-14 nm | 12-14 nm | 12-14 nm | 12-14 nm |
| Purity | >90% | >90% | >90% | >90% | >90% |
| Form | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder |
| Available Size | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg |
| Shipping | Room Temperature | Room Temperature | Room Temperature | Room Temperature | Room Temperature |
| Storage | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. |
| Catalog | N-MNP-15 | N-MNP-16 | N-MNP-17 | N-MNP-18 |
|---|---|---|---|---|
| Product Name | Mouse MSP2N2 with a his-tag | Human MSP2N2 with a his-tag | Mouse MSP2N2, tag-free | Human MSP2N2, tag-free |
| Species | Mouse | Human | Mouse | Human |
| Tag | N-Terminal His-tag | N-Terminal His-tag | Tag-free | Tag-free |
| Scaffold Diameter | ~17 nm | ~17 nm | ~17 nm | ~17 nm |
| Purity | >90% | >90% | >90% | >90% |
| Form | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder |
| Available Size | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg | 2 mg; 10 mg |
| Shipping | Room Temperature | Room Temperature | Room Temperature | Room Temperature |
| Storage | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. | Stored lyophilized powder at -20°C. Stored reconstituted solution at 2-8°C. |
Alfa Chemistry is pleased to offer membrane scaffold protein (MSP) products to help our customers conduct their research and achieve more. We guarantee that all our MSP products are of high quality that can be well used in membrane protein study. The MSP products listed above are some of the classic and current standard products at Alfa Chemistry. Please contact us if you cannot find the MSP products you want in our product catalogue, we also can offer customized product with specific properties designed in on a contract or collaborative basis.
Our products and services are for research use only.
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