A remarkable advancement in the development of membrane-mimetic systems was the advent of lipid-bilayer nanodiscs, which initially were assembled with the aid of membrane scaffold proteins (MSPs) and, more recently, using synthetic polymer. The diisobutylene/maleic acid (DIBMA) copolymer is composed of alternating diisobutylene and maleic acid moieties, which has been found as useful polymer in synthetic nanodiscs to solubilize cell membranes. It was found that DIBMA is able to solubilise several different membrane proteins from different expression systems. In addition, compared with poly(styrene-co-maleic acid) (SMA), a polymer commonly used in synthetic nanodiscs, DIBMA displays a higher tolerance to divalent cations, and is non-absorbance at 280 nm due to the presence of aliphatic rather than aromatic hydrophobic pendant groups in the structure.
Alfa Chemistry offers a wide range of DIBMA products with different polymer chains lengths and different buffer solutions. In addition, we provide various modified DIBMA. The variety of options of DIBMA will be ideal tools for your membrane protein solubilization of interest.
Fig. 1. The structure of DIBMA.
Our DIBMA products can be roughly divided into following three series.
01. This series of products with different polymer chains lengths are lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5.
| Product Name | DIBMA (10000) lyophilized from HEPES | DIBMA (10000) lyophilized from TRIS | DIBMA (12000) lyophilized from HEPES | DIBMA (12000) lyophilized from TRIS |
|---|---|---|---|---|
| Catalog | N-SNP-13 | N-SNP-14 | N-SNP-15 | N-SNP-16 |
| Usage | Protein solubilization | Protein solubilization | Protein solubilization | Protein solubilization |
| Formula Weight | 10000 g/mol | 10000 g/mol | 12000 g/mol | 12000 g/mol |
| pH | 7.5 in buffer | 7.5 in buffer | 7.5 in buffer | 7.5 in buffer |
| dn/dc | 1.35 M-1 | 1.35 M-1 | 1.35 M-1 | 1.35 M-1 |
| Solubility | >10 % in H2O | >10 % in H2O | >10 % in H2O | >10 % in H2O |
| Absorbance at 280 nm | <0.3 (1 % solution) | <0.3 (1 % solution) | <0.3 (1 % solution) | <0.3 (1 % solution) |
| Divalent Cationic Tolerance | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs |
| Available Specification | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g |
02. This series of products are modified DIBMA, which have a reduced charge to drastically reduce the affinity to the ions. This was achieved by adding glucosamine or an amino-functionalized diol to DIBMA. This modified DIBMA can be used for experiments for which the presence of ions. This series of products are lyophilized from two different buffer solutions (HEPES or TRIS).
| Product Name | DIBMA Glucosamine lyophilized from HEPES | DIBMA Glucosamine lyophilized from TRIS | DIBMA Glycerol lyophilized from HEPES | DIBMA Glycerol lyophilized from TRIS |
|---|---|---|---|---|
| Structure |
|
| ||
| Catalog | N-SNP-17 | N-SNP-18 | N-SNP-19 | N-SNP-20 |
| Usage | Protein solubilization | Protein solubilization | Protein solubilization | Protein solubilization |
| Formula Weight | ~10000 or 12000 g/mol | ~10000 or 12000 g/mol | ~10000 g/mol | ~10000 g/mol |
| pH | 7.5 in buffer | 7.5 in buffer | 7.5 in buffer | 7.5 in buffer |
| dn/dc | 1.35 M-1 | 1.35 M-1 | 1.35 M-1 | 1.35 M-1 |
| Solubility | >10 % in H2O | >10 % in H2O | >10 % in H2O | >10 % in H2O |
| Absorbance at 280 nm | <0.3 (1 % solution) | <0.3 (1 % solution) | <0.3 (1 % solution) | <0.3 (1 % solution) |
| Divalent Cationic Tolerance | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs | Dependend on DIBMA product Increased with less charged DIBMAs |
| Available Specification | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g |
03. This series of products are a modified version of DIBMA. Glyco-DIBMA is a modified with an added sugar side-chain to the DIBMA polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. Sulfo-DIBMA is an electroneutral modification of existing DIBMAs and polymerized by RAFT. It does not interfere with charge-sensitive interactions between proteins and lipids, and can stay stable in the wide pH range.
| Product Name | Glyco-DIBMA | Sulfo-DIBMA |
|---|---|---|
| Structure |
| - |
| Catalog | N-SNP-21 | N-SNP-22 |
| Full Name | - | Poly(diisobutylene-alt-N´,N´-/dimethyl(maleimidopropyl))ammonium propane sulfonate copolymer, sodium salt in 50 mM HEPES, pH 7.5 |
| Physical State | Lyophilized powder, to be solved with water | Lyophilized powder to be solved with water |
| Usage | Membrane protein solubilization & stabilization | |
| Formula Weight | 15701 g/mol | 8000 g/mol ± 1000 g/mol |
| pH | 7.5 after solving | 7.5 ± 0.3 (upon addition of dH2O) |
| Total pH Stability | - | 4-10 |
| Recommended Concentration Range Upon Use | - | 1% to 5% |
| Solubility | >10% | >10 % in H2O |
| Divalent Cationic Tolerance | <4 mM | - |
| Absorbance at 280 nm | >0.3 (1 % solution) | <0.3 (1 % solution) |
| Buffer | HEPES | 50 mM HEPES |
| Specific Gravity | 1.1 | - |
| Mg2+ Tolerance | - | over 100 mM |
| Ca2+ Tolerance | - | over 100 mM |
| Color | - | White to slightly yellow |
| Odor | - | Odorless |
| Available Specification | 10 x 50 mg, 1g, 10 x 1 g | 10 x 50 mg, 1g, 10 x 1 g |
Unless otherwise specified, the shipping temperature of all products is 2-8 °C, and the products can be stored at -20 °C for several years in lyophilized powder form and at 2-8 °C for several days when dissolved.
Alfa Chemistry is pleased to offer DIBMA products to help our customers conduct their research and achieve more. We guarantee that all our DIBMA products are of high quality that can be well used in membrane protein study. In addition to the DIBMA products listed above, we can offer novel variants and derivatives with specific properties designed in on a contract or collaborative basis. If there is any need, please contact us.
Our products and services are for research use only.
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