Membrane Proteins from Detergent Platform

The demand for membrane protein grows rapidly with the development of modern drug research, and traditional preparation methods of membrane protein can not meet the needs of modern drug research and development. In addition, the preparation of membrane proteins faces many challenges, including low expression, poor stability, low solubility and difficult purification of membrane proteins. Alfa Chemistry is dedicated to the development of membrane proteins. In order to produce high quality and high purity membrane protein products, we have successfully built a detergent technology platform based on insect cell expression technology and mammalian cell expression technology, which effectively improves the yield and purity of membrane protein. The principle of detergent technology platform is as follows: The transmembrane region of membrane proteins (especially transmembrane proteins) is highly hydrophobic, making membrane proteins is difficult to maintain the correct conformation in ordinary buffers. In addition, membrane proteins are very tightly bound to the cell membrane and difficult to dissolve in solution. Detergent is an amphiphilic molecule with both hydrophilic polar groups and hydrophobic non-polar groups in its structure, which can extract membrane proteins from the cell membrane, as well as can provide a hydrophobic environment for membrane proteins in solution, thereby maintaining the hydrophobic transmembrane structure of membrane proteins.