Human GPRC5D Protein, His & FLAG Tag (Nanodisc)

Catalog	DGS-MPNP-1
Purity	≥75% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized recombinant Human SMA-GPRC5D Protein at 5 μg/mL (100 μL/well) can bind Anti-Human GPRC5D Antibody, Human IgG1, the EC50 is 2-6ng/mL. 2. Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind GPRC5D Protein with an affinity constant of 0.782 nM as determined in BLI assay (Routinely tested). 3. Captured Anti-GPRC5D antibody on anti-Human IgG Fc via CM5 Chip can bind GPRC5D protein with an affinity constant of 9.609nM as determined in a SPR assay (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.
Molecular Mass	32.83 kDa
Predicted N Terminal	Met
Protein Construction	A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
Shipping	Dry ice
Species	Human