Human CLDN6 Protein, His & FLAG Tag (Nanodisc)

Catalog	DGS-MPNP-2
Purity	≥90% as determined by SDS-PAGE
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Loaded Anti-CLDN6 antibody on ProA Biosensor, can bind Human CLDN6 Protein with an affinity constant of 0.536nM as determined in BLI assay (Routinely tested). 2. Captured Anti-Human CLDN6 antibody on CM5 Chip via anti-human IgG Fc antibody, can bind Recombinant Human CLDN6 Protein, His & FLAG Tag (Nanodisc) with an affinity constant of 7.03 nM as determined in a SPR assay (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.
Molecular Mass	The recombinant human CLDN6 consists of 202 amino acids and has a predicted molecular mass of 21.87 kDa. The apparent molecular mass of it is approximately 20.91 KDa in SDS-PAGE under reducing conditions.
Predicted N Terminal	Met 1
Protein Construction	A DNA sequence encoding the human CLDN6 (P56747) (Met1-Thr184) was expressed, with a Flag tag followed by a polyhistidine tag at the C-terminus.
Shipping	Dry ice
Species	Human