Human CD133 Full Length Protein, His Tag (Nanodisc)

Catalog	DGS-MPNP-8
Synonyms	CD133, PROM1, PROML1, Prominin-1, AC133
Purity	>90% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized Human CD133 Full Length Protein, His Tag (Nanodisc) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested). 2. 2e5 of Anti-CD133 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human CD133 Full Length Protein, His Tag (Nanodisc) and negative control protein respectively, washed and then followed by PE-anti-His Tag antibody and analyzed with FACS (QC tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Molecular Mass	The protein has a calculated MW of 97.2 kDa and migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted N Terminal	Gly 20
Protein Construction	The CD133 carries a polyhistidine tag at the C-terminus.
Shipping	Dry ice
Species	Human