Human CCR5 Protein, Flag & His Tag (Detergent)

Catalog	DGS-MPDP-3
Synonyms	CCR5, CMKBR5, CD195, CC-CKR-5, CHEMR13
Purity	>85% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized Human CCR5 Protein, Flag & His Tag at 10 μg/mL (100 μL/well) can bind Purified NA/LE Mouse Anti-Human CD195 with a linear range of 0.078-2.5 μg/mL (QC tested). 2. Human CCR5 Protein, Flag & His Tag captured on CM5 chip via anti-His antibody can bind Anti-CCR5 antibody (Human IgG1) with an affinity constant of 0.0737 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested). 3. Purified NA/LE Mouse Anti-Human CD195 (Mouse IgG2a) captured on CM5 chip via anti-mouse antibodies surface can bind Human CCR5 Protein, Flag & His Tag with an affinity constant of 7.51 nM as determined in a SPR assay (in presence of DDM and CHS) (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Molecular Mass	The protein has a calculated MW of 46.0 kDa. The protein migrates as 33-35 kDa and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Predicted N Terminal	Asp
Shipping	Dry ice
Species	Human