Human A2aR Protein, His & Flag Tag (Nanodisc)

Catalog	DGS-MPNP-7
Purity	≥95% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized Human A2aR Protein, His & Flag Tag (Nanodisc) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ADORA2A antibody, Mouse IgG2a, the EC50 is 1.3-5.2ng/mL.
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as sterile 10mM Hepes, 50mM NaCl, pH 7.5.
Molecular Mass	The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal	Asp 1
Protein Construction	A DNA sequence encoding the human AA2AR (P29274) (Pro2-Leu208 & Glu219-Ala316) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
Shipping	Dry ice
Species	Human