Fluorescent Human Claudin-6 Full Length Protein, GFP & His Tag (Nanodisc)

Catalog	DGS-MPNP-4
Purity	≥80% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Recombinant Fluorescent Human Claudin-6 Full Length Protein, GFP & His Tag (Nanodisc) at 5 μg/mL (100 μL/well) can bind Anti-Human Claudin6 Antibody, Human IgG1, the EC50 is 8-24 ng/mL.
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as sterile 10mM Hepes, 50mM NaCl, 1% glycerin, pH 8.0.
Molecular Mass	The recombinant Human CLDN6-Nanodisc consists of 220 amino acids and predicts a molecular mass of 23.3 kDa. It migrates as an approximately 37.2 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal	Met 1
Protein Construction	A DNA sequence encoding the Human CLDN6(P56747-1) (Met1-Val220) was expressed with a C-terminal GFP followed by a His tag.
Shipping	Dry ice
Species	Human