FITC-Labeled Human GPRC5D Protein, Flag & His Tag (Nanodisc)

Catalog	DGS-MPNP-17
Synonyms	GPRC5D
Purity	>80% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized FITC-Labeled Human GPRC5D Protein, Flag & His Tag (Nanodisc) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPRC5D antibody, Human IgG4 with a linear range of 0.2-16 ng/mL (QC tested). 2. Anti-GPRC5D Antibody, Human IgG4 captured on Protein A Chip can bind FITC-Labeled Human GPRC5D Protein, Flag & His Tag (Nanodisc) with an affinity constant of 9.29 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Molecular Mass	The protein has a calculated MW of 51.8 kDa. The protein migrates as 19-27 kDa, 36-37 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted N Terminal	Asp
Protein Construction	This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.
Shipping	Dry ice
Species	Human