Biotinylated Human GPRC5D Protein, His & Avitag & Flag Tag (Nanodisc)

Catalog	DGS-MPNP-16
Synonyms	GPRC5D
Purity	>85% as determined by SDS-PAGE.
Storage	Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized Biotinylated Human GPRC5D Protein, His & Avitag & Flag Tag (Nanodisc) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Mouse IgG1 with a linear range of 0.002-0.313 μg/mL (QC tested). 2. Anti-GPRC5D Antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human GPRC5D Protein, His & Avitag & Flag Tag (Nanodisc) with an affinity constant of 105 nM as determined in a SPR assay (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Molecular Mass	The protein has a calculated MW of 53.5 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE).
Predicted N Terminal	Asp
Protein Construction	This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag.
Shipping	Dry ice
Species	Human