Biotinylated Human CLDN9 Protein, His & AVI Tag (Nanodisc)

Catalog	DGS-MPNP-5
Purity	>80% as determined by SDS-PAGE.
Storage	Samples are stable for up to12 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity	1. Immobilized Biotinylated Human CLDN9 Protein, His & AVI Tag (Nanodisc) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL,100 μL/well) can bind Anti-Human Claudin9 Antibody, Human IgG1,the EC50 is 10-60 ng/mL. 2. Loaded Biotinylated Human CLDN9 Protein, His & AVI Tag (Nanodisc) on SA Biosensor, can bind Anti-CLDN9 antibody with an affinity constant of 11.15 nM as determined in BLI assay (Routinely tested).
Endotoxin	Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host	HEK293 Cells
Formulation	Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.
Molecular Mass	22.44 kDa
Predicted N Terminal	Met 1
Protein Construction	A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Shipping	Dry ice
Species	Human