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Biotinylated Human Claudin-18.2 Protein, Avitag & His Tag (Nanodisc)

Catalog DGS-MPNP-13
Synonyms Claudin-18.2, CLDN18, Claudin-18
Storage Samples are stable for up to 3 months from date of receipt at -70 °C. Store it under sterile conditions at -70 °C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity 1. Immobilized Biotinylated Human Claudin-18.2 Protein, Avitag & His Tag (Nanodisc) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
2. Biotinylated Human Claudin-18.2 Protein, Avitag & His Tag (Nanodisc) captured on Biotin CAP-Series S Sensor Chip can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 (Cat. No. CL2-M34) with an affinity constant of 0.953 nM as determined in a SPR assay(Routinely tested).
Endotoxin Less than 1.0 EU per μg protein as determined by the LAL method.
Expressed Host Baculovirus-Insect cells.
Formulation Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Molecular Mass 24.8 kDa
Predicted N Terminal Met
Protein Construction This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag
Shipping Dry ice
Species Human

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