MSP Nanodisc Products

Application

The research team used Alpaca MSP1D1 with a His-tag. After binding with the membrane protein, MSP1D1 successfully formed a stable nanodisc system. During affinity chromatography, the team extracted and purified the membrane protein from 2L of culture medium, obtaining approximately 5 mg of purified protein with over 95% purity. With the support of MSP1D1, the membrane protein remained stable at room temperature for 72 hours without losing activity, providing an ideal sample for subsequent structural analysis.

Results

The research team resolved the membrane protein's 3D structure using single-particle cryo-electron microscopy (cryo-EM). The results showed that the nanodisc system with Alpaca MSP1D1 achieved a resolution of 3.5 Å, significantly better than the 4.8 Å resolution obtained using traditional methods, enhancing the accuracy of structural analysis. This structural data provided a reliable foundation for downstream drug screening and functional studies of the membrane protein.

Customer Feedback

"We are extremely satisfied with the performance of Alpaca MSP1D1. It not only excels in stabilizing membrane proteins but also, with the His-tag, greatly simplifies protein purification and significantly improves our experimental efficiency. The final resolved structure achieved even higher resolution than we anticipated."

Application

The company chose Mouse MSP1D1-His with POPC. This product uses mouse-derived MSP1D1 combined with the phospholipid POPC to form a highly stable nanodisc system. By incorporating the target membrane protein into this system, the research team employed surface plasmon resonance (SPR)-based drug screening to identify potential small-molecule inhibitors. During the initial screening, 30 small molecules were tested, and 5 compounds showed significant inhibitory activity. The most potent inhibitor exhibited an IC50 of 15 nM.

Results

Using this nanodisc system, the team quickly obtained effective drug candidates and validated their binding to the membrane protein. The results demonstrated that Mouse MSP1D1-His with POPC increased the sensitivity of the drug screening process, reduced screening time by over 40%, and enabled the discovery of multiple highly potent candidate molecules.

Customer Feedback

"We used Mouse MSP1D1-His with POPC for membrane protein drug screening, and the results were excellent. The system was highly stable, and the membrane protein's functionality was perfectly preserved, significantly improving our screening efficiency. We successfully identified several potential inhibitors with IC50 values far below our previous expectations.""

Application

The company utilized the Human MSP1D1 dH5-tag-free, DMPC Nanodisc Assembly Kit. This tag-free MSP1D1 protein, combined with DMPC lipids, efficiently assembled into stable nanodiscs. The research team incorporated the target membrane protein into these nanodiscs and used Förster Resonance Energy Transfer (FRET) to assess the protein-ligand binding affinity. The experiments showed that using this system, the membrane protein exhibited a binding constant (Kd) of 2.5 nM, which was significantly more accurate compared to results without the nanodisc system (Kd = 8.7 nM).

Results

Through this system, researchers successfully obtained high-affinity binding data for the target membrane protein and its ligand and carried out ligand screening experiments. Preliminary results indicated that the binding affinity increased approximately threefold, and the membrane protein maintained functional stability for 72 hours within the nanodisc system.

Customer Feedback

"The Human MSP1D1 dH5-tag-free, DMPC Nanodisc Assembly Kit has been invaluable for our membrane protein functional studies. The tag-free design preserves the natural function of the protein, allowing us to accurately measure ligand-protein interactions. Our data show a significant increase in binding affinity, providing critical insights for our subsequent drug screening efforts."