Anionic Detergents Line

Purchased Product

Sodium dodecyl sulfate (SDS, CAS 151-21-3)

Application Scenario

The laboratory used high-purity SDS supplied by Alfa Chemistry for SDS-PAGE protein electrophoresis experiments. In the study of membrane protein solubilization and protein quantification, SDS disrupts the secondary and tertiary structures of proteins while imparting a uniform negative charge, allowing proteins to be accurately separated according to molecular weight.

Experimental Data

• Protein extraction using 1% (w/v) SDS buffer achieved an extraction efficiency of over 95%
• In SDS-PAGE experiments, protein molecular weight separation error was less than ±2 kDa
• Repeated experiments (n=5) showed a standard deviation of less than 5%, demonstrating high reproducibility

Customer Feedback

"The quality of SDS from Alfa Chemistry is extremely consistent. Every batch produced uniform experimental results, significantly reducing the time spent on repeated experiments. The high-purity product has greatly facilitated our membrane protein research."

Purchased Product

Sodium Lauryl Ether Sulfate (SLES)

Application Scenario

The R&D team used SLES for cell lysis and biological sample preparation, facilitating the solubilization of cell membranes and release of intracellular proteins and nucleic acids. In combination with buffer systems, SLES ensured uniform cell lysis and did not interfere with downstream enzyme activity assays or proteomic analyses.

Experimental Data

• 1.5% (w/v) SLES was used for HeLa cell lysis, achieving a protein recovery rate of 92%
• Enzyme activity retention in downstream ELISA assays >90%
• Repeated cell lysis experiments (n=4) showed a standard deviation of less than 6%

Customer Feedback

"The high solubility and mild properties of SLES allowed us to obtain reliable data in our drug screening experiments. The technical team also provided usage recommendations, saving us significant optimization time."

Purchased Products

Mixed anionic detergents (SDS + SLES combination)

Application Scenario

The research team used the anionic detergent system to solubilize and disperse proteins, lipids, and polysaccharides in plant extract samples, optimizing the purification process of natural products. By controlling detergent concentration and processing conditions, they ensured stable and reliable results in downstream HPLC analysis.

Experimental Data

• Using a 0.8% SDS + 0.5% SLES mixed system, extraction efficiency increased by approximately 20%
• Target compound purity reached 98% in HPLC analysis
• Standard deviation of three consecutive batch experiments was<3%, ensuring high reproducibility

Customer Feedback

"The mixed detergents performed very well, producing clear and reproducible HPLC results. Alfa Chemistry’s technical support team also helped us optimize the extraction conditions, which was extremely helpful for our natural product research."